Nutrient medium (Ham) were originally developed to support the growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa cells and mouse L cells. Both mixtures were formulated for use with or without addition of serum, depending on the cultured cell type.
Ham’s F10 is used to support the growth of Chinese Hamster Ovary cells under serum free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.
Ham’s F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.
Ham’s F14 Medium was elaborated from our Ham’s F12 which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham’s F14 contains a double concentration of amino acids compared to the Ham’s F12. This product is also supplemented with Calcium Chloride and Ascorbic Acid.