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Ham's Nutrient Medium

Category: Synthetic Cell Culture Media

Ham's Nutrient Medium (F10 , F12, and F14)

Nutrient medium (Ham) was originally developed to support the growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa cells and mouse L cells. Both mixtures were formulated for use with or without the addition of serum, depending on the cultured cell type.

Ham's F10

Ham’s F10 is used to support the growth of Chinese Hamster Ovary cells under serum free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Code: LM-H1030

Product: Ham’s F10 w/ L-Glutamine w/25mM Hepes

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 12 months

Size:Cat Number :
500 ml
LM-H1030/500

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Download technical datasheet >

Download formulation document >

Code:  LM-H1040

Product: Ham’s F10 w/ L-Glutamine

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 12 months

Size:Cat Number :
500 ml
LM-H1040/500

Download technical datasheet >

Download safety datasheet >

Download formulation document >

Code: LM-H1045

Product: Ham’s F10 w/o L-Glutamine

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 12 months

Size:Cat Number :
500 ml
LM-H1045/500

Download technical datasheet >

Download safety datasheet >

Download formulation document >

Code: PM-H1046

Product: Ham’s F10 w/ L-Glutamine w/o Sodium Bicarbonate

Volume: 1 litre / 10 litres

Sterility: Non-strerile

Storage: +2/+8°C

State: Powder

Shelf Life: 36 months

Size:Cat Number :
1 litre
PM-H1046/1
10 litres
PM-H1046/10

Download technical datasheet >

Download safety datasheet >

Download formulation document >

Ham's F12

Ham’s F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

Code:  LM-H1235

Product:  Ham’s F12 w/ L-Glutamine

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 12 months

Size:Cat Number :
500 ml 
LM-H1235/500

Download technical datasheet >

Download safety datasheet >

Download formulation document >

Code:  LM-H1236

Product:  Ham’s F12 w/o L-Glutamine

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 24 months

Size:Cat Number :
500 ml 
LM-H1236/500

Download technical datasheet >

Download safety datasheet >

Download formulation document >

Code:  PM-H1234

Product: Ham’s F12 w/ L-Glutamine w/o Sodium Bicarbonate

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Powder

Shelf Life: 36 months

Size:Cat Number :
1 L
PM-H1234/1
10 L
PM-H1234/10

Download technical datasheet >

Download safety datasheet >

Download formulation document >

Ham's F14

Ham’s F14 Medium was elaborated from our Ham’s F12 which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham’s F14 contains a double concentration of amino acids compared to the Ham’s F12. This product is also supplemented with Calcium Chloride and Ascorbic Acid.

Code:  LM-H1438

Product: Ham’s F14 w/ 6g/L Glucose w/ 1mg/L ATP

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 24 months

Size:Cat Number :
500 ml
LM-H1438/500

Download technical datasheet >

Download safety datasheet >

Download formulation document >

Ham's F10

Product

Ham’s F10 w/ L-Glutamine w/25mM Hepes

Code: LM-H1030

Product: Ham’s F10 w/ L-Glutamine w/25mM Hepes

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 12 months

Size:Cat Number :
500 ml
LM-H1030/500
Product

Ham's F10 w/ L-Glutamine

Code:  LM-H1040

Product: Ham’s F10 w/ L-Glutamine

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 12 months

Size:Cat Number :
500 ml
LM-H1040/500
Product

Ham's F10 w/o L-Glutamine

Code: LM-H1045

Product: Ham’s F10 w/o L-Glutamine

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 12 months 

Size:Cat Number :
500 ml
LM-H1045/500
Product

Ham's F10 w/ L-Glutamine w/o Sodium Bicarbonate

Code: PM-H1046

Product: Ham’s F10 w/ L-Glutamine w/o Sodium Bicarbonate

Volume: 1 litre / 10 litres

Sterility: Non-strerile

Storage: +2/+8°C

State: Powder

Shelf Life: 36 months

Size:Cat Number :
1 litre
PM-H1046/1
10 litres
PM-H1046/10

Ham's F12

Product

Ham's F12 w/ L-Glutamine

Code:  LM-H1235

Product:  Ham’s F12 w/ L-Glutamine

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 12 months

Size:Cat Number :
500 ml
LM-H1235/500
Product

Ham's F12 w/o L-Glutamine

Code:  LM-H1236

Product:  Ham’s F12 w/o L-Glutamine

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 24 months

Size:Cat Number :
500 ml
LM-H1236/500
Product

Ham's F12 w/ L-Glutamine w/o Sodium Bicarbonate

Code:  PM-H1234

Product: Ham’s F12 w/ L-Glutamine w/o Sodium Bicarbonate

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Powder

Shelf Life: 36 months

Size:Cat Number :
1 L
PM-H1234/1
10 L
PM-H1234/10

Ham's F14

Product

Ham's F14 w/ 6g/L Glucose w/ 1mg/L ATP

Code:  LM-H1438

Product: Ham’s F14 w/ 6g/L Glucose w/ 1mg/L ATP

Volume: 500 ml

Sterility: Sterile-filtered

Storage: +2/+8°C

State: Liquid

Shelf Life: 24 months

Size:Cat Number :
500 ml
LM-H1438/500