Medium 199

This complex medium was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.

The media can be formulated either with Earle salts or Hanks Salts. The media formulated with the Earle salts are buffered with a bicarbonate / carbonic acid solution and retain their pH in a CO2 incubator. The use of the Earle salts under ambient conditions results in a rapid rise in the pH of the culture medium.

The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions and their use in a CO2 incubator results in a rapid drop in the pH of the culture medium.

Code
Product
Volume
LM-M1901
Medium 199 w/ Hanks' Salts w/ L-Glutamine
500ml
Sterility : Sterile filtered
State : Liquid
Storage : +2/+8°C
Shelf Life : 18 months
Size :
500ml
Cat Number :
LM-M1901/500
LM-M1903
Medium 199 w/ Earle's Mod. Salts w/ L-Glutamine w/ 1.25g/l Sodium Bicarbonate
500ml
Sterility : Sterile filtered
State : Liquid
Storage : +2/+8°C
Shelf Life : 18 months
Size :
500ml
Cat Number :
LM-M1903/500
LM-M1904
Medium 199 w/ Earle's Salts w/o L-Glutamine
500ml
Sterility : Sterile filtered
State : Liquid
Storage : +2/+8°C
Shelf Life : 24 months
Size :
500ml
Cat Number :
LM-M1904/500
PM-M1906
Medium 199 modified w/ Hanks' Salts w/o L-Glutamine w/o Sodium Bicarbonate
1L/10L
Sterility : Non sterile
State : Powder
Storage : +2/+8°C
Shelf Life : 36 months
Size :
1L
10L
Cat Number :
PM-M1906/1
PM-M1906/10
PM-M1907
Medium 199 w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate
1L/10L
Sterility : Non sterile
State : Powder
Storage : +2/+8°C
Shelf Life : 36 months
Size :
1L
10L
Cat Number :
PM-M1907/1
PM-M1907/10
PM-M1908
Medium 199 w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes
1L/10L
Sterility : Non sterile
State : Powder
Storage : +2/+8°C
Shelf Life : 36 months
Size :
1L
10L
Cat Number :
PM-M1908/1
PM-M1908/10
LM-M1909
Medium 199 w/ Earle's Salts w/ Stable Glutamine w/ 25 mM Hepes
500ml
Sterility : Sterile filtered
State : Liquid
Storage : +2/+8°C
Shelf Life : 24 months
Size :
500ml
Cat Number :
LM-M1909/500