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The irrigating buffers and salt solutions are sterile physiological balanced solutions intended for the use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. They were used for the first steps for preparing parts of organs and during the dissociation process or for isolation of cell suspensions. Further applications are the intermediate steps for cultivation of cells like washing centrifugation, suspending and counting and also many analytical methods for FACS, t solutions should be used to keep the cells only for minutes or a maximum of a few hours into suspension. These buffers and solutions are not cell culture media. They are composed of a phosphate buffer system, sodium chloride to adjust the osmolarity and perhaps sugar and metal ions for a short time for nutrition and stabilisation of morphology. For applications where Ca2+ and Mg2+ ions interfere with enzyme activity e.g. Trypsin, use the modified HBSS products.
CODE
P2066
P2010
P2015
P2025
P2029
P2065
P2069
P2073
P2076
P2079
P2088
P2090
P2092
P2099
P2092
PRODUCT
Cupric Sulphate Pentahydrate
Ferric Nitrate Nonahydrate
Ferric Sulphate Heptahydrate
Magnesium Sulphate Anhydrous
Magnesium Sulphate Anhydrous
Sodium Chloride
Sodium Phosphate Monobasic Anhydrous
Sodium Phosphate Monobasic Dihydrate
Sodium Phosphate Dibasic Anhydrous
Sodium Phosphate Dibasic Heptahydrate
Zinc Sulphate Heptahydrate
Calcium Chloride Dihydrate
Calcium Chloride Anhydrous
Calcium Nitrate Tetrahydrate
Calcium Chloride Anhydrous
CODE
L0180
L0600
L0601
L0602
L0605
L0606
L0607
L0608
L0609
L0611
L0612
L0615
L0625
L0636
L0640
L0642
L0643
L0680
PRODUCT
HEPES Buffer 1M
Earle's Balanced Salt Solution (EBSS) w/o phenol red w/o Ca w/o Magnesium Sulphate
EBSS w/o Ca w/o Magnesium Sulohate
EBSS w/Ca w/Magnesium Sulphate
Hank's Balanced Salt Solution (HBSS) w/o Ca w/o Mg w/o phenol red
HBSS w/Ca w/Mg w/Na Bicarbonate w/phenol red
HBSS
w/o Mg w/o Ca w/Na Bicarbonate w/o phenol red
HBSS w/o Na Bicarbonate w/phenol red
HBSS w/o Mg w/o Ca w/o Na Bicarbonate w/phenol red
HBSS HBSS w/o Mg w/o Ca w/ Na Bicarbonate w/phenol red
HBSS w/o phenol red
Dulbecco's Phosphate Buffered Saline (DPSS) w/o Mg w/o Ca Chloride
Dulbecco's PBS w/ Mg w/ Ca Chloride
Alsever's Soln
NaCl Salt Soln 0.85%
Na Pyruvate 100mM
Potassium Chloride 0.07mM
Na Bicarbonate 7.5%
CODE
X0112
X0113
X0507
X0509
X0510
X0512
X0513
X0515
X0520
PRODUCT
Earle's Balanced Salt Solutions (EBSS) 10X concentrate
EBSS 10X concentrate, w/o Na Bicarbonate, w/o Ca w/o Mg
Hank's balanced Salt Solutions (HBSS) 10X w/o phenol red w/o Ca w/o Mg, w/o Na Bicarbonate
HBSS 10X concentrate w/o Na Bicarbonate, w/phenol red
HBSS 10X concentrate w/o phenol red w/o Ca w/o Mg w/Na Bicarbonate
HBSS 10X concentrate w/phenol red w/o Ca, w/o Mg
HBSS 10X w/o Ca w/o Mg w/o Na Bicarbonate w/phenol
Phosphate Buffered Saline (PBS) 10X concentrate w/o Mg w/o Ca
PBS 10X concentrate
CODE
P0153
P0154
P0750
P0751
P5455
PRODUCT
Hanks Buffered Salt Solution (HBSS) w/o Ca w/o Mg w/o Na Bicarbonate
HBSS w/Ca w/Mg w/o Na Bicarbonate
Dulbecco's Phosphate Buffered Saline (DPBS) w/o Na Bicarbonate w/o Ca w/o Mg
DPBS w/Ca w/Mg
HEPES