The irrigating buffers and salt solutions are sterile physiological balanced solutions intended for the use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. They were used for the first steps for preparing parts of organs and during the dissociation process or for isolation of cell suspensions. Further applications are the intermediate steps for cultivation of cells like washing centrifugation, suspending and counting and also many analytical methods for FACS, t solutions should be used to keep the cells only for minutes or a maximum of a few hours into suspension. These buffers and solutions are not cell culture media. They are composed of a phosphate buffer system, sodium chloride to adjust the osmolarity and perhaps sugar and metal ions for a short time for nutrition and stabilisation of morphology. For applications where Ca2+ and Mg2+ ions interfere with enzyme activity e.g. Trypsin, use the modified HBSS products.