Biosera media formulations are manufactured following 
original publications, standards set by the Tissue 
Culture Association and accepted formulations. These 
formulations may vary from these standards by 
substituting hydrated, chlorinated and/or the salt forms 
of certain compounds where such substitutions 
contribute to improved performance of the product.

The media formulations are computerised and each 
batch is generated for weight and batch number. All 
of the chemicals, raw materials and equipment that 
we use is of the highest quality. All new batches of 
chemicals are introduced in the process after 
stringent QC.

The water used for media preparation is of the best 
quality. It is of WFI quality, produced in several steps including centrifugal distillation. The 
conductivity is measured in line and the water is always freshly processed and cooled down 
to 20 °C before adding the powder.

Batch sizes for powdered media range from 1 to 10,000 Litres, for liquid media 
from 1 to 2,000 Litres.

Samples of powder and liquid media are tested physically for osmotic effects and pH. 
Osmolarity is determined by means of the freezing point method. The chemical composition and 
the homogeneity of the mixture of the components are controlled by analysis of glucose in the 
sample. Biological performance testing is done on different cell lines. Endotoxin testing is 
performed using the Limulus Amoebocyte test. 

Powdered media are stable for 3 years when stored at 2-8 °C in their original containers and in 
dry, dark conditions. Heat, light and humidity can greatly effect the performance of powdered 
media therefore we recommend that powder be stored correctly if not used entirely. All liquid 
media formulation. are stable for up to 2 years. They must be stored at 2-8 °C in the dark. 
Media concentrates are stored at 8-10 °C and are stable for 1 year at the original pH.