It was realised at an early stage the overall potential of vitamins not only in the field of research but also for therapeutic benefits of humans. The specialised field of science concerning cell biology discovered the great importance for Vitamins for In Vitro cultivation of cells. It was discovered that mammalian cells are not able to synthesise this class of biological compounds and the artificial basic cell culture media containing sugars, salts and amino acids were supplemented with the different isolated vitamins. These formulations were later intensified. The development of nutritional media without the need for sera as a source of undefined growth promoting components ensured all vitamins are now introduced into media formulations. It has become more and more clear that each cell type needs its individual medium as it is diverted from the different qualities of different organs from different species.