What is the best way to thaw serum ?

Serum is thawed at 2-8°C to prevent degradation; alternatively, it may be thawed at room temperature with periodic agitation to resuspend the contents.

Thawed serum should be mixed thoroughly before being added to cell culture medium.

Temperatures above 37°C can compromise serum performance by accelerating the degradation of critical serum factors, and should be avoided in the lab.

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