Is it necessary to heat inactivate serum?

For most cell culture applications, heat inactivation of serum is not recommended. Heat inactivation degrades complement proteins that may interfere with immunological assays. Heating serum for prolonged periods of time can reduce or destroy growth factors, as well as increase the formation of deposits which are commonly mistaken for microbial contamination.

Filed under:

Comment on this FAQ

Your email address will not be published. Required fields are marked *