Glasgow MEM

Glasgow Minimum Essential Medium was originally developed by Ian MacPherson and Michael Stoker as a modification of Eagle’s medium (BME). This medium was used to study the genetic factors affecting cell competence : Polyoma virus was used to transform four fibroblast clones from a culture of baby hamster kidney (BHK-21) cells.

The modifications to the BME are:
* adding 10% tryptose phosphate
* twice the normal concentration of amino acids and vitamins.

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