Some preparations of trypsin contain phenol red. Since the product is shipped with dry ice, there could be a significant CO2 build up in packaging. This CO2 may enter the solution and lower the pH slightly, giving an orange (around pH 6.5) vs. pinkish (around 7.3) color. The solution, if orange (acidic) can still be good to… Read more »
Except where notified on product labels, Biosera sera and liquid media are sterile filtered. Sterility is not guaranteed after opening. The use of aseptic manipulation techniques is recommended.
Serum is thawed at 2-8°C to prevent degradation; alternatively, it may be thawed at room temperature with periodic agitation to resuspend the contents. Thawed serum should be mixed thoroughly before being added to cell culture medium. Temperatures above 37°C can compromise serum performance by accelerating the degradation of critical serum factors, and should be avoided in… Read more »
Turbidity and flocculent material may be present after thawing or after prolonged freezing and/or refrigeration. Our experience indicates these modifications do not affect the biological performance of the serum. Some deposits are due to fibrin: Our collection procedures and rapid freezing may leave some fibrinogen in the serum. Fibrinogen is the soluble precursor of the clot-forming… Read more »
Serum may be stored between +2 to +8°C for up to 8 weeks without diminishing its performance (*). Internal studies in Biowest have shown that during 26 weeks of storage (between +2 and +8°C) the serum’s capacity to promote cell growth was not significantly altered. (*) Art to Science, Vol.19, No.2, “Serum Stability at Refrigerated… Read more »
The serum should be stored frozen and protected from light. The recommended storage temperature is -10°C to -40°C. At temperatures below -40°C, the bottles may become brittle increasing the risk of breakage.
According to the OIE (World Animal Health Organization), there is no risk of BSE being transmitted in several products, including: milk, hides and skins, deboned meat, blood and blood by-products, such as fetal bovine serum (OIE Terrestrial Code 11.4 BSE). Most OIE member countries have adopted this position, including the European Union and the United States (USDA)…. Read more »
Bovine spongiform encephalopathy (BSE), more commonly known as “mad cow” disease, is a fatal neurodegenerative disease (encephalopathy) in cattle
Biosera tests the serum for the viruses (BVD, IBR, PI3) by using a cell culture method, so that live viruses, if present, are detected in the serum.
The sampling for sterility tests of all Biowest products conforms to the European Pharmacopeia requirements.