At standard dosage of gamma irradiation, no loss of performance is observed on the first generations of cells. We notice no change on hormones, osmolarity, pH, endotoxins nor electrophoretic profile of the FBS. As ionizing radiations kill viruses, it also break some proteins and decolorate the product by reducing haemoglobin level. Radiations also decrease the serum metabolites (ALP, ALT, AST, LDH) and… Read more »
FBS is sometimes gamma irradiated to eliminate all potential virus risks.
-Start by thawing the serum and the control serum bottle at 2-8°C. -Stir the bottled serum to avoid a precipitant from forming during the thawing, then place it in the water bath adjusted to 80°C. -Once the control bottle temperature reaches 50°C, adjust the bath thermostat to 70°C; when the bottle temperature reaches 55°C, change… Read more »
For most cell culture applications, heat inactivation of serum is not recommended. Heat inactivation degrades complement proteins that may interfere with immunological assays. Heating serum for prolonged periods of time can reduce or destroy growth factors, as well as increase the formation of deposits which are commonly mistaken for microbial contamination.