Trypsin
Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of “anchorage-dependent ” mammalian cells and tissues.
The concentration of Trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.
- Recombinant Trypsin
Recombinant Trypsin is a genetically engineered protein expressed in E-Coli.
As such it is totally animal free, free from contaminating enzymes like chymotrysin as well as protease inhibitors. It is highly stable with a high purity (95%). It is widely used in insulin manufacturing, vaccines and cell culture applications.
- Accutase
Accutase is a “ready to use” cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is the “gentler form” of Trypsin. It can replace Trypsin/EDTA for the detachment and dissociation of “anchorage-dependent” cells from surfaces.
It can also be used on suspension cells to reduce clumping in preparation for counting.
- Lymphosep
Lymphosep is designed for a simple, rapid isolation of lymphocytes from whole blood, once diluted and treated with anti-coagulant or defibrinating agent.
- Cell Culture Water
Cell Culture Water is suitable to dissolve dry powder cell culture media in accordance to the technical data sheet.
Sterility tests include aerobic and anaerobic bacteriological flora, fungis and yeast. The endotoxin level is less than 0.005EU / mL
- Bioguard
Bioguard is a disinfectant solution for incubators, water baths and surfaces in daily use in cell culture and molecular biology laboratories.
- Glutamine
L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-Glutamine can degrade relatively rapidly.
- Amino Acids and Vitamins
Our solution of concentrated amino acids, vitamins or non essential amino acids can be added to enrich your basal medium, enabling it to be used as a classical MEM or BME.
The final concentration of the BME or MEM must be 1X.
- Colcemid
Colcemid prevents spindle formation responsible for cell division during the mitosis process, thereby permitting an increase in number of metaphases.
- Phytohaemagglutinin M -PHA-M
PHA-M is a lectin extracted from red kidney beans (Phaseolus vulgaris). PHA-M is the mucoprotein form and is a crude extract used for the stimulation of cell proliferation in lymphocyte culture. PHA-M also has a powerful erythroagglutinating property and it was originally used for separating leukocytes from whole blood.
- D-Glucose Monohydrate – Dextrose, Cell Culture Tested
D-Glucose Monohydrate is a common natural sugar involved in processes, such as energy production, glycosylation, and formation of glycans that provide structure to cells.
It is involved in a detrimental process in cells called glycation.
It is used as a supplement for cell culture and in numerous cellular processes.
